Silybum Marianum Research Articles

Silymarin Improves Thyroid Function in Lithium-treated Bipolar Patients: A Randomized, Double-blind, Placebo-controlled Pilot Study

Background: Thyroid dysfunction is one of the most important side effects of lithium carbonate. Silymarin is a flavonolignan extracted from the milk thistle Silybum marianum (L.), which has remarkable antioxidant and therapeutic properties. This clinical trial study aimed to evaluate the effect of silymarin on thyroid function and serum antioxidant status in patients with lithium-treated bipolar disorder. Methods: Bipolar patients with a depression episode and a history of at least six months of lithium use were randomly divided into placebo-control (n=18) and intervention (n=19) groups. In addition to standard medication, patients in the intervention and control groups received silymarin (140 mg) and placebo tablets daily for ten weeks, respectively. Finally, thyroid function and serum antioxidant status were evaluated along with clinical signs at the beginning and the tenth week. Results: Following the administration of silymarin, a significant increase was observed in total antioxidant capacity (p = 0.004) and total thiol molecules (p = 0.005) levels in serum compared to the placebo group. Although silymarin had no significant effect on serum triiodothyronine (T3) and lithium levels, it could significantly improve the secretory status of thyroid-stimulating hormone (TSH; p = 0.002) and Thyroxine (T4; p = 0.02) hormones in comparison to the placebo group. Conclusion: The current study showed that silymarin might be effective for thyroid function in lithium- treated bipolar patients by improving body’s antioxidant status. Trial Registration Number: IRCT20190811044513N2

Exploring the hepatoprotective effects of silymarin in experimentally induced- diclofenac sodium toxicity in rabbits

The study was aimed to investigate the hepatoprotective effects of varying doses of silymarin against liver damage caused by diclofenac sodium in rabbits. Silymarin, extracted from the Silybum marianum plant, is known for its antioxidant properties and is used to treat liver diseases. The rabbits (n=40) were divided into 4 groups. Group A served as the control (with vehicle solution), group B, as toxin control with diclofenac sodium (50mg/kg intra-peritoneally), group C received both diclofenac sodium and protective dose of silymarin (50mg/kg orally), while group D was administered diclofenac sodium along with higher dose of silymarin (100mg/kg orally). At the study's conclusion, rabbits were humanely euthanized and blood samples were collected for analysis. Enzymes such as Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), direct bilirubin, serum urea, and total bilirubin were measured. The study revealed that higher dose of silymarin (100mg/kg) was more effective in reducing serum enzyme levels compared to lower dose, indicating stronger hepatoprotective effect. This suggests silymarin's potential as the therapeutic agent against liver damage and toxicity induced by substances like diclofenac sodium

Nutraceutical Aspects of Selected Wild Edible Plants of the Italian Central Apennines

All over the world, wild edible plants are an essential source of chemical components that justify their use in folk medicine. The aim of this review is to document and summarize the knowledge of ten wild plants analyzed in a previous study for their ethnomedical significance. Achillea millefolium, Borago officinalis, Foeniculum vulgare, Gentiana lutea, Juniperus communis, Laurus nobilis, Malva sylvestris, Satureja montana, Silybum marianum and Urtica dioica were the subjects of our study. They are commonly found in the central Italian Apennines and the Mediterranean basin. Phytochemicals contained in wild plants, such as phenols, polyphenols, flavonoids, condensed tannins, carotenoids, etc., are receiving increasing attention, as they exert a wide range of biological activities with resulting benefits for human health. Based on the 353 studies we reviewed, we focused our study on the following: (a) the ethnobotanical practices and bioactive phytochemicals; (b) the composition of polyphenols and their role as antioxidants; (c) the methodologies commonly used to assess antioxidant activity; (d) the most advanced spectroscopic and spectrometric techniques used to visualize and characterize all components (metabolomic fingerprinting). The potential of pure compounds and extracts to be used as nutraceuticals has also been highlighted through a supposed mechanism of action.

Effect of silver nanoparticles formulated by Silybum marianum on menopausal urinary incontinence in ovariectomized rats

Abstract Urinary incontinence is the major sign of menopause genitourinary syndrome and is usually related to the sexual dysfunctions. Fifty percent of menopausal women have menopausal urinary tract. Menopause genitourinary syndrome is a hypoestrogenic condition with external sexual, urological, and genital implications. The production of estrogen decline after menopause is an important parameter for urinary incontinence. Recently, nanotechnology has been applied as a remedial option for the genitourinary disease treatment. Silver nanoparticles were mediated by the Silybum marianum aqueous extract for the menopausal urinary incontinence treatment in ovariectomized rats in the current experiment. The green-formulated AgNPs@Silybum marianum were characterized by FT-IR, TEM, SEM, and UV–Vis. The SEM findings prove the spherical morphology with size of 15–60 nm. The ovariectomized rats were treated by AgNPs@Silybum marianum (5 and 20 µg/kg/day) for 56 days. According to the results, AgNPs@Silybum marianum increased the urinary bladder weight and reduced the body weights in comparison with the untreated rats. AgNPs@Silybum marianum rats indicated a dose-dependent amelioration for the acetylcholine contraction index. AgNPs@Silybum marianum also ameliorated the levels of serum 17β-estradiol, urinary bladder hydroxyproline, triglyceride, cholesterol, LDL, HDL, ALP, AST, and ALT in the ovariectomized rats. Based on the experiment results, the recent formulation may be applied for the menopausal urinary incontinence treatment in humans after performing the clinical research.

Chromosome-level genome assembly of milk thistle (Silybum marianum (L.) Gaertn.)

Silybum marianum (L.) Gaertn., commonly known as milk thistle, is a medicinal plant belonging to the Asteraceae family. This plant has been recognized for its medicinal properties for over 2,000 years. However, the genome of this plant remains largely undiscovered, having no reference genome at a chromosomal level. Here, we assembled the chromosome-level genome of S. marianum, allowing for the annotation of 53,552 genes and the identification of transposable elements comprising 58% of the genome. The genome assembly from this study showed 99.1% completeness as determined by BUSCO assessment, while the previous assembly (ASM154182v1) showed 36.7%. Functional annotation of the predicted genes showed 50,329 genes (94% of total genes) with known protein functions in public databases. Comparative genome analysis among Asteraceae plants revealed a striking conservation of collinearity between S. marianum and C. cardunculus. The genomic information generated from this study will be a valuable resource for milk thistle breeding and for use by the larger research community.

Potential Protective Effects of Milk Thistle (Silybum Marianum L.) Seeds Against Benzo[a]Pyrene-Induced Hepatic and Nephritic Injuries in Rats: Biochemical and Histopathological Studies.

Potential Protective Effects of Milk Thistle (Silybum Marianum L.) Seeds Against Benzo[a]Pyrene-Induced Hepatic and Nephritic Injuries in Rats: Biochemical and Histopathological Studies.

Tunisian Silybum Species: Important Sources of Polyphenols, Organic Acids, Minerals, and Proteins across Various Plant Organs.

Silybum marianum and Silybum eburneum are wild edible Mediterranean plants used in the human diet. This study presents the initial findings on the phytochemical characterization of Tunisian S. marianum and S. eburneum organs. It examined their mineral, sugar, organic acid, polyphenolic, and seed storage protein contents, as well as their antioxidant potential. In S. marianum, stems had high sodium and potassium contents, while the immature and mature seeds were rich in calcium and magnesium. However, S. eburneum had high potassium levels in stems and high sodium and calcium levels in the flowers. S. marianum showed substantial fructose variation among its organs. Conversely, S. eburneum exhibited significant heterogeneity in glucose, sucrose, and maltose levels across its organs, with maltose exclusively detected in the immature seeds. A notable organ-dependent distribution of organic acids was observed among the two species. Higher levels of phenolic contents were detected in both mature and immature seeds in both species compared to the other plant parts. The seeds possessed higher antioxidant activities than other plant organs. In both S. marianum and S. eburneum seeds, albumins and globulins were the predominant protein fractions. This study brings evidence supporting the important potential of Silybum organs as sources of nutrients with antioxidant properties for producing functional food.

THE USE OF RABBIT MEAT AND MILK THISTLE MEAL IN A LOW-CALORIE MEAT PRODUCT

In the modern world, the culture of a healthy lifestyleacquiresspecial relevance. An integral part of this process is specialized nutrition, therapeutic and prophylactic or dietary consumption of meat products for different categories of people. According to the research of modern scientists, in the next 15-20 years, the consumption of therapeutic and prophylactic, dietary, low-calorie meat products, as well as a decrease in the energy value of food products, will occupy 30% of the food market.The research results were processed by mathematical analysis and compiled taking into account the nutritional value and organoleptic properties of the finished product, which includes rabbit and veal meat, rabbit liver, egg mellange, skimmed milk, milk thistle powder. The combination of animal products in various proportions makes it possible to bring the composition of the product closer to the composition recommended by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO).This article provides an overview of the main components of the composition for obtaining a low-calorie meat product that meets current and future market demand, rabbit meat and milk thistle powder, known for their hepatoprotective properties, analyzes the usefulness of their composition for human health and suggests the possibility of inclusion in the daily diet. In addition, a laboratory study of the nutritional and energy value of the finished product was conducted, as a result of which a decrease in the caloric content of the new product to 138 kcal was observed.Keywords: rabbit meat, dietary nutrition, low-calorie meat product, alatykene powder, amino acids, energy value.

Nephroprotective effects of silymarin and its fabricated nanoparticles against aluminum-induced oxidative stress, hyperlipidemia, and genotoxicity.

Aluminum (Al) is a ubiquitous element with proven nephrotoxicity. Silymarin (SM) is a mixture of polyphenolic components extracted from Silybum marianum and exhibited protective influences. However, SM bioactivity can be enhanced by its incorporation in chitosan (CS) through the use of nanotechnology. This work proposed to assess the protective influence of SM and its loaded chitosan nanoparticles (SM-CS-NPs) on aluminum chloride (AlCl3)-induced nephrotoxicity. Six groups were created randomly from 42 male Wistar rats and each one contains 7 rats (n = 7). Group I, acted as a control and received water. Group II received SM (15 mg/kg/day) and group III administered with SM-CS-NPs (15 mg/kg/day). Group IV received AlCl3 (34 mg/kg) and groups V and VI were treated with SM and SM-CS-NPs with AlCl3 respectively for 30 days. AlCl3 administration significantly elevated TBARS, H2O2, and kidney function levels besides LDH activity. Whereas GSH, CAT, SOD, GPx, GST, and GR values were all substantially reduced along with protein content, and ALP activity. Additionally, significant alterations in lipid profile, hematological parameters, and renal architecture were observed. Moreover, TNF-α, TGF-β, and MMP9 gene expression were upregulated in kidney tissues. The administration of SM or its nanoparticles followed by AlCl3 intoxication attenuated renal dysfunction replenished the antioxidant system, and downregulated TNF-α, TGF-β, and MMP9 gene expression in renal tissues compared to the AlCl3 group. SM-CS-NPs have more pronounced appreciated protective effects than SM and have the proficiency to balance oxidant/antioxidant systems in addition to their anti-inflammatory effect against AlCl3 toxicity.

Dietary supplementation of micelle silymarin enhances the antioxidant status, innate immunity, growth performance, resistance against Vibrio parahaemolyticus infection, and gut morphology in Pacific white shrimp (Litopenaeus vannamei)

Silymarin, a flavonoid compound derived from the milk thistle plant (Silybum marianum) is well-known for its hepatoprotective, antioxidant, immunomodulatory, and anti-inflammatory properties. Therefore, this study sought to evaluate the effect of silymarin and micelle silymarin (MS) supplementation on Pacific white shrimp (Litopenaeus vannamei). The experimental diets were formulated to include MS at levels of 0.25, 0.5, 1.0, 2.0, and 4.0 g/kg or silymarin at levels of 1.0 and 2.0 g/kg in the basal diet (Con, M0.25, M0.5, M1, M2, M4, S1 and S2, respectively). Juvenile shrimp (0.98 g) were fed with experimental diets (four replicates) for 42 days. The growth metrics were significantly (P < 0.05) increased in M0.25, M0.5, and M1 groups compared to the Con group. Feed conversion ratio decreased (P < 0.05) in M0.5, M1, and M2 groups, whereas the protein efficiency ratio increased (P < 0.05) in M0.5 and M1 groups compared to the Con group. Phenoloxidase, lysozyme, glutathione peroxidase, and superoxide dismutase activities were enhanced (P < 0.05), with the highest levels observed in the M0.5 group. Malondialdehyde level decreased (P < 0.05) in M1 and M2 groups compared to the Con group. Total protein increased (P < 0.05) in M0.25, M0.5 and S1 groups compared to the Con group. AST levels were significantly reduced (P < 0.05) in shrimp fed M0.5, M1, M2, M4, and S2 groups, and ALT levels decreased (P < 0.05) in M1 and S2 groups compared to Con group. Wall thickness was significantly higher (P < 0.05) in the M0.5 group, and villi length increased (P < 0.05) in M0.25, M0.5, and S1 groups. The resistance against Vibrio parahaemolyticus increased; however, no significant differences in cumulative survival were observed (P ˃ 0.05). Significantly higher (P < 0.05) proPO levels were observed in M0.5, M1, M2, M4, and S2 groups, whereas higher (P < 0.05) TLR-3 levels were observed in M1 and M2 groups compared to the Con group. TNF-α expression decreased (P < 0.05) in all other dietary treatments compared to the Con group. The findings suggest that dietary silymarin and MS can enhance growth, immune response, antioxidant capacity, gut morphology, and immune gene expression, while reducing lipid peroxidation and inflammation in Pacific white shrimp. The shrimp performance shows no significant difference between silymarin and MS groups at 1% and 2% levels. The optimal suggested inclusion level of MS in shrimp feed is 0.5–1.0 g/kg.

Protective Effect of Silibinin on Lipopolysaccharide-Induced Endotoxemia by Inhibiting Caspase-11-Dependent Cell Pyroptosis.

To explore the protective effect and the underlying mechanism of silibinin (SIB), one of the active compounds from Silybum marianum (L.) Gaertn in endotoxemia. Mouse peritoneal macrophage were isolated via intraperitoneally injection of BALB/c mice with thioglycolate medium. Cell viability was assessed using the cell counting kit-8, while cytotoxicity was determined through lactate dehydrogenase cytotoxicity assay. The protein expressions of interleukin (IL)-1 α, IL-1 β, and IL-18 were determined by enzyme-linked immunosorbent assay. Intracellular lipopolysaccharide (LPS) levels were measured by employing both the limulus amoebocyte lysate assay and flow cytometry. Additionally, proximity ligation assay was employed for the LPS and caspase-11 interaction. Mice were divided into 4 groups: the control, LPS, high-dose-SIB (100 mg/kg), and low-dose-SIB (100 mg/kg) groups (n=8). Zebrafish were divided into 4 groups: the control, LPS, high-dose-SIB (200 εmol/L), and low-dose-SIB (100 εmol/L) groups (n=30 for survival experiment and n=10 for gene expression analysis). The expression of caspase-11, gasdermin D (GSDMD), and N-GSDMD was determined by Western blot and the expressions of caspy2, gsdmeb, and IL-1 β were detected using quantitative real-time PCR. Histopathological observation was performed through hematoxylineosin staining, and protein levels in bronchoalveolar lavage fluid were quantified using the bicinchoninicacid protein assay. SIB noticeably decreased caspase-11 and GSDMD-mediated pyroptosis and suppressed the secretion of IL-1 α, IL-1 β, and IL-18 induced by LPS (P<0.05). Moreover, SIB inhibited the translocation of LPS into the cytoplasm and the binding of caspase-11 and intracellular LPS (P<0.05). SIB also attenuated the expression of caspase-11 and N-terminal fragments of GSDMD, inhibited the relative cytokines, prolonged the survival time, and up-regulated the survival rate in the endotoxemia models (P<0.05). SIB can inhibit pyroptosis in the LPS-mediated endotoxemia model, at least in part, by inhibiting the caspase-11-mediated cleavage of GSDMD. Additionally, SIB inhibits the interaction of LPS and caspase-11 and inhibits the LPS-mediated up-regulation of caspase-11 expression, which relieves caspase-11-dependent cell pyroptosis and consequently attenuates LPS-mediated lethality.

Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure

The study examined the potential of Silymarin, a blend of bioactive flavonolignans extracted from the milk thistle Silybum marianum, to mitigate Deltamethrin-induced toxicity in the blood of Cyprinus carpio. Fish were exposed to Deltamethrin (0.66 μg/L), the plant extract, or a combination of both for a duration of thirty days. Various parameters, including serum biochemical markers, erythrocytic abnormalities, and genotoxicity endpoints, were assessed. Results indicated a significant (p < 0.05) increase in the levels of AST, ALT, ALP, blood urea nitrogen, creatinine, glucose, cholesterol, and TLC in the fish exposed to the pesticide. Conversely, total protein, TEC, and Hb showed a notable decrease. There was also a notable rise in micronuclei and erythrocytic abnormalities such as acanthocytes, microcytes, and notched cells. Under ultrastructural examination, phenotypic deformities like spherocytosis, discocytes, and clumped erythrocytes were observed. However, dietary supplementation of silymarin (1 g/kg) significantly restored the biochemical, genetic, and cellular parameters, resembling those of the control group. This suggests the potential of this plant extract in protecting the common carp, Cyprinus carpio, from Deltamethrin-induced damage by scavenging free radicals and reducing DNA oxidative stress.

Rifampin- and Silymarin-Mediated Pharmacokinetic Interactions of Exogenous and Endogenous Substrates in a Transgenic OATP1B Mouse Model.

Organic anion-transporting polypeptides (OATP) 1B1 and OATP1B3, encoded by the SLCO gene family of the solute carrier superfamily, are involved in the disposition of many exogenous and endogenous compounds. Preclinical rodent models help assess risks of pharmacokinetic interactions, but interspecies differences in transporter orthologs and expression limit direct clinical translation. An OATP1B transgenic mouse model comprising a rodent Slco1a/1b gene cluster knockout and human SLCO1B1 and SLCO1B3 gene insertions provides a potential physiologically relevant preclinical tool to predict pharmacokinetic interactions. Pharmacokinetics of exogenous probe substrates, pitavastatin and pravastatin, and endogenous OATP1B biomarkers, coproporphyrin-I and coproporphyrin-III, were determined in the presence and absence of known OATP/Oatp inhibitors, rifampin or silymarin (an extract of milk thistle [Silybum marianum]), in wild-type FVB mice and humanized OATP1B mice. Rifampin increased exposure of pitavastatin (4.6- and 2.8-fold), pravastatin (3.6- and 2.2-fold), and coproporphyrin-III (1.6- and 2.1-fold) in FVB and OATP1B mice, respectively, but increased coproporphyrin-I AUC0-24h only (1.8-fold) in the OATP1B mice. Silymarin did not significantly affect substrate AUC, likely because the silymarin flavonolignan concentrations were at or below their reported IC50 values for the relevant OATPs/Oatps. Silymarin increased the Cmax of pitavastatin 2.7-fold and pravastatin 1.9-fold in the OATP1B mice. The data of the OATP1B mice were similar to those of the pitavastatin and pravastatin clinical data; however, the FVB mice data more closely recapitulated pitavastatin clinical data than the data of the OATP1B mice, suggesting that the OATP1B mice are a reasonable, though costly, preclinical strain for predicting pharmacokinetic interactions when doses are optimized to achieve clinically relevant plasma concentrations.

Antioxidant Effect of a Combination of S-Acetyl-L-Glutathione, Vitamin E, Silybum Marianumon Hepatic Cells under Oxidative Stress: An In Vitro Study

Oxidative stress plays a key role in the pathogenesis of liver diseases and can be involved in the inflammatory process of liver cells. The aim of this vitro study is to assess the antioxidant efficacy of three distinct components (fermented S-Acetyl -L-glutathione, Silybum marianum (L.) Gaern. and vitamin E all-rac-alpha-tocopheryl acetate) both individually and in combination (Glutasyl product). In addition, we also evaluated the combined antioxidant effect of the three ingredients on human hepatic cells subjected to oxidative stress induced by H2O2 treatment. The results showed the dose-dependent antioxidant potential of the three components suggesting promising applications in medical contexts. The synergistic antioxidant effects observed for the Glutasyl was more than the individual components. Additionally, Glutasyl showed not-toxic activity on human hepatocytes and it could be considered useful in mitigating cytotoxicity induced by oxidative stress.

Genetic activity and cytotoxic effect of Silybum marianum extract in comparison with the impact of antitumor drugs etoposide and methotrexate: a study on Drosophila melanogaster

One of the most important directions in medical practice involves the exploration of protectors and adaptogens to mitigate side effects associated with the use of antitumor and potent medications. The extracts of medicinal plants are actively tested as protectors. In connection with this, the aim of the research was to determine the effectiveness of the protective properties of Silybum marianum extract against the drugs ‘etoposide’ and ‘methotrexate’ at concentrations of 800 μg/kg using the model object Drosophila melanogaster. To analyse the protective properties, the following research methods were applied: determining the lethal concentration, average individual fertility of individuals, mortality of F1 offspring at embryonic stages, SMART (a method for determining the frequency of somatic mutations and recombinations) and assessing the intensity of programmed cell death. The study revealed that a 5% S. marianum extract reduces the genotoxicity of etoposide and exhibits a high cytotoxic effect, both independently and in combination with etoposide and methotrexate.

Abstract 7307: Silibinin regulates glucocorticoid signaling via mast cells: Potential treatment of androgen-resistant prostate cancer

Abstract Prostate cancer (PCa) is the second most common cancer in American men. The American Cancer Society estimates that in 2023 there have been ~288,300 new cases of PCa and ~34,700 deaths from PCa in the United States. We previously showed that silibinin (SB; a natural flavonolignan from milk thistle seeds) is an effective therapeutic against PCa that inhibits cell growth and mitogenic cell survival via EGFR, IGFR-1, and NFκB signaling. However, the effect of SB on the tumor immunological microenvironment has not been elucidated. Mast cells (MCs) are archetypal solider of the immune system. The dynamics of PCa and MCs are complex, where they may contribute to tumor transformation/progression/regression; furthermore, their role in PCa is poorly understood. To address this gap and understand the role of SB in MC regulation, MCs were derived from the bone marrow (BMMCs) of C57BL/6 mice. BMMCs were generated in the presence of IL-3 and SCF, and were confirmed to be BMMCs using flow cytometry (dual staining: cKit/Fc€RI). Thereafter, the BMMCs were exposed to different doses of SB (25-100 µM) and two concentrations (25 µM; SB 25 and 100 µM; SB 100) were selected for further experiments. BMMCs treated with SB 25, SB 100, and untreated (control) were subjected to proteomic analysis using LCMS on Fusion Lumos mass spectrometer. A total of 3575 proteins were identified using the annotated mouse proteome. Statistical analysis was performed using ANOVA followed by Fisher’s posthoc analysis (p&amp;lt;0.05, FDR&amp;lt;0.01) and 166 proteins were found to be have statistically significant differential expression amongst the three groups (n=4/group). These 166 proteins were selected for further pathway analysis using Ingenuity Pathway Analysis (IPA; Qiagen). Only pathways specifically demonstrated experimentally in prostatic tissues/cells were selected for further analysis. Important nodes found for SB regulation of MCs in PCa were androgen receptor (AR), transmembrane serine protease (TMPRSS), and NK3 Homeobox 1 (NKX3-1). AR, TMPRSS, and NkX3-1 are well established in PCA. Most important pathway found was glucocorticoid receptor (GR) signaling; nuclear receptor subfamily 3 group C member 1 (NR3C1) and HS90 were the most important proteins identified in GR signaling. GR signaling may cause androgen deprivation therapy (ADT)-resistance by directly activating AR-target genes (including TMPRSS2) and/or activating an independent transcriptome. NR3C1 has been shown to share target specificity with AR and is highly upregulated in the resistant tumors. Thus, SB could play an important therapeutic role, specifically in ADT-resistant tumors via regulation of MCs. Further studies are warranted to delineate these mechanisms. Understanding these mechanistic could also potentially aid to decipher a general mechanism of resistance to antiandrogens and potential of SB in treating ADT-resistant tumors. Citation Format: Neha Mishra, Sandeep Paudel, Komal Raina, Paul Maroni, Chapla Agarwal, Rajesh Agarwal. Silibinin regulates glucocorticoid signaling via mast cells: Potential treatment of androgen-resistant prostate cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 7307.

Abstract 7310: Dietary feeding of silibinin effectively protects against UVB-induced basal cell carcinoma

Abstract Exposure to UVB radiation causes various malignancies, including basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). BCC is the most prevalent skin cancer type worldwide; ~3.6 million cases of BCC are diagnosed annually in the USA. Silibinin (SB) is a naturally occurring flavonolignan that is present in milk thistle seeds. It has been shown to effectively reduce BCC occurrence upon UVB radiation exposure, in both pre- and post-treatment studies, when applied topically. However, SB efficacy, when fed in diet, against UVB-induced BCC formation and growth has not been reported. We addressed this question employing Ptch+/- mice, where animals were divided into three groups. Group 1: control group, mice were fed adjusted vitamin diet (AIN-76A; TD94096) with no UVB exposure. Group 2: UVB group, mice were fed pellet control diet (semi-purified, modified AIN-76A) and exposed to UVB (240 mJ/cm2) 3 times/week (Mon, Wed, Fri). Group 3: SB group, mice were fed 1% SB (w/w)-supplemented diet and exposed to UVB similar to that in Group 2. This protocol was followed for 26 weeks; thereafter, animals were sacrificed at 14 weeks and 26 weeks post-UVB initiation, and skin samples were harvested and analyzed for BCC formation. β-galactosidase staining showed the development of BCC lesions at 26 weeks in the UVB group; however, SB-fed group had both lesser number of BCC lesions and decreased area covered by BCCs as compared to the UVB group. Non-UVB exposed control group had no BCC lesions. To address if SB feeding starts exerting its efficacy earlier than 26 weeks, we selected the early efficacy timepoint for transcriptomics analysis to determine the mechanism of SB efficacy. Skin RNA samples from the 14-week time point (n=4/group; RIN≥7; DV200&amp;gt;85%) were subjected to library preparation (550 ng of total RNA; Zymo-Seq RiboFree Total RNA Library Kit), ribosomal RNA-depletion RNA sequencing (80 million paired-end reads/sample), and data processing. About 16,000 unique genes were identified, of which ~65% were protein-coding genes. The samples within each group were highly correlated (mean&amp;gt;0.99; Pearson’s correlation). The UVB cluster was sequestered away from the other groups, indicating that the SB diet effectively mitigated the effects of UVB exposure, even at the transcriptome level. Additionally, gene ontology and pathway enrichment analysis (Ingenuity Pathway Analysis, Qiagen) of differentially expressed genes (p&amp;lt;0.05; fold change≤2) showed UVB-induced aberration and SB-associated enrichment in mitotic prometaphase, metaphase, and anaphase pathways, RHO GTPase signaling, and cell cycle regulation; the top functional network found to be dysregulated was lipid metabolism. Taken together, these results highlight the potential of dietary SB intake (apart from topical SB) to be an effective modality against UVB-induced BCC formation and growth and identify molecular targets that are plausibly involved in SB efficacy. Citation Format: Sandeep Paudel, Neha Mishra, Komal Raina, Chapla Agarwal, Rajesh Agarwal. Dietary feeding of silibinin effectively protects against UVB-induced basal cell carcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 7310.

Impact of pre-partum nutraceutical or monensin intraruminal boluses on colostrum quality and Holstein dairy cows’ performance: exploratory field study

A smooth transition phase is the key for optimal dairy cows’ performance and reduced antibiotics use. Therefore, the objective of our Our study was to compare the effect of an antibiotic growth promoter and a nutraceutical bolus, onhealth, colostrum, milk production, and profitability in transition dairy cows. Seventy-five animals blocked by parity, previous milk yield, lactation length, and body condition score (BCS) were assigned in a randomised design to 1 of 3 groups: control (CON; N = 26) receiving no treatment; monensin (MON; N = 27) receiving, a slow-releasing intraruminal bolus of sodic monensin at −20 d relative to expected calving; nutraceutical (ECS; N = 22) receiving, at −5 d before the expected calving an intraruminal bolus of Echinacea purpurea dry extract, vitamin E, l-carnitine and Silybum marianum released within 24h. Colostrum yield and density were recorded; its composition and immunoglobulins content were analysed. Every 20 days BCS was recorded; individual milk yield was recorded daily until 70DIM and monthly thereafter until 305 DIM. Milk quality was tested monthly. An economic evaluation until 70DIM was performed. Colostrum and Δ BCS were analysed by analysis of covariance. Economics and BCS were analysed with ANOVA, and milk yield and quality with ANOVA for repeated measures. The MON and ECS had lower colostrum protein and His, Arg, Ala, Met, Val and Ile content, and in higher milk yield until 35 (ECS) and 70 (MON) DIM compared to the CON. A trend for a higher partial income was observed for MON and ECS compared to the CON group, suggesting a positive impact of the treatments.

The first record of milk thistle (Silybum marianum, Asteraceae) as an alien invasive weed species in Addis Ababa City, Ethiopia.

Abstract Since 2020, milk thistle Silybum marianum, an invasive weed species, has been widely observed in urban green spaces and roadsides in Addis Ababa city, Ethiopia. To plan future management activities, this study aimed to determine the current distribution status of the weed in urban green areas of Addis Ababa city. Weed surveys were conducted across 42 urban green sites located in Yeka, Lemi Kura, Bole, Nifas Silk-Lafto, Kolfie Keraniyo, Akaky Kality, and Gullele sub-cities during the main rainy season from June to September 2023. Urban green areas were selected using purposive sampling techniques. Field observations confirmed that Silybum marianum can invade and colonize a wide range of habitats and is highly competitive, posing a threat to important native plant species. High weed density rates and dense stands of weeds were recorded in the 34 urban green areas surveyed. The survey results strongly suggest implementing fast integrated weed management options such as mowing/tilling, weeding before the plant goes to seed, cutting (stems and/or seed heads), and burning (collected plant parts and/or seed heads) into a prepared burrow to minimize the spread of the weed and its ecological impacts. The purpose of this survey study is to provide information about Silybum marianum to minimize or avoid its distribution and associated impacts by following sustainable invasive weed management programs in the city before spreading to other cities in the country.

Ethnobotanical study of Silybum marianum (L.) Gaertn in the Taounate Region (Northern Morocco): Knowledge and Use of the Plant for Food and Medicinal Purposes

Ethnobotanical study of Silybum marianum (L.) Gaertn in the Taounate Region (Northern Morocco): Knowledge and Use of the Plant for Food and Medicinal Purposes